Revelation 1:4 John, to the seven churches which are in Asia: Grace to you and peace from Him who is and who was and who is to come, and from the seven Spirits who are before His throne.
John employs the first use of the mention of 7 in Revelation. This number, which Biblically signifies completion, occurs 54 times in this book; twice in this verse alone. The choice of using the number seven for the churches John writes to may have particular significance.